阳春砂仁ITS2序列PCR反应体系的优化及验证
摘要
模板和引物5个因素进行优化,结果表明20μL阳春砂仁ITS2序列最佳PCR反应体系包括TaqDNA聚合酶0.80U、dNTPs0.20mmol·L-1、Mg2+2.50mmol·L-1、DNA模板40ng、引物0.20umol·L-1,验证实验表明优化体系稳定性强,可用于后续的DNA条
形码分析。
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["[1] u8d39 u5e0c u540c , u5de8 u82d7 u82d7 , u6797 u6e90 , u7b49 .ITS2 u5e8f u5217 u5728 u690d u7269","DNA u6761 u5f62 u7801 u9274 u5b9a u4e2d u7684 u5e94 u7528 ( u7efc u8ff0 )[J]. u4e9a u70ed u5e26 u690d u7269 u79d1 u5b66 ","2014,43(4):339-342","[2] u9648u58ebu6797 , u5b8bu7ecfu5143 , u59dau8f89 , u7b49 . u836fu7528u690du7269 .DNA u6761u5f62u7801u9274","u5b9au7b56u7565u53cau5173u952eu6280u672fu5206u6790 [J]. u4e2du56fdu5929u7136u836fu7269 ,2009,7(5): 322-","327. ","[3] u5218u68a6u695a . u57fau4e8eu201cu8fa8u72b6u8bbau8d28u201du53cau6c14u3001u5473u6570u5b57u5316u7684u7802u4ec1u836f","u6750u8d28u91cfu8bc4u4ef7u7814u7a76 [D]. u5e7fu5ddeu4e2du533bu836fu5927u5b66 ,2017.","[4] u6768u5f69u971e . u4e91u5357u4ea7u9633u6625u7802u4ec1u4e0eu8fdbu53e3u7802u4ec1u5316u5b66u6210u5206u53cau4e3bu8981","u836fu6548u5b66u7684u6bd4u8f83u7814u7a76 [D]. u4e91u5357u4e2du533bu5b66u9662uff0c2017.","[5]Chen S, Yao H, Han J,etal.Validation of the ITS2 ","Region as a Novel DNA Barcode for Identifying Medicinal ","Plant Species[J].plos ONEuff0c2010,5(1):e8613.","[6]u5218u8273 .u9633u6625u7802u7ec4u7ec7u57f9u517bu4e0eu8f90u5c04u8bf1u53d8u80b2u79cdu7684u521du6b65u7814u7a76 [D].","u5e7fu5ddeu4e2du533bu836fu5927u5b66 ,2010.","[7] u4f55u6b63u6587 , u5218u8fd0u751f , u9648u7acbu534e , u7b49 . u6b63u4ea4u8bbeu8ba1u76f4u89c2u5206u6790u6cd5","u4f18u5316 PCR u6761u4ef6 [J]. u6e56u5357u533bu79d1u5927u5b66u5b66u62a5 ,1998.23(4):403-404.","[8] u5192u7ef4u7ef4 , u9a6cu91d1u9a8f , u8584u5929u5cb3 , u7b49 . u6b63u4ea4u8bbeu8ba1u4f18u5316u83dcu85b9","ISSR u53cdu5e94u4f53u7cfbu7814u7a76 [J]. u5206u5b50u690du7269u80b2u79cd ,2006,4(6): 137-141.","[9] u738bu4e3du4fca , u738bu971e . u957fu67f1u91d1u4e1du6843 ISSR-PCR u53cdu5e94u4f53u7cfbu7684u5efau7acb","u4e0eu4f18u5316 [J]. u4e1cu5317u6797u4e1au5927u5b66u5b66u62a5 ,2017,45(10):13-16."]
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